AST is widely used clinically to determine antibiotic resistance patterns of bacterial which are isolated from various clinical specimens, to guide antibiotic treatment decisions, and predict therapeutic outcome.
For performing antimicrobial susceptibility testing a pure culture of the pathogen is required, isolation of pathogens may take several days, sometimes the entire test needs to be repeated, or setting up additional tests, this delays the diagnosis of resistant bacteria and decisions for appropriate and effective antibiotic therapy. such delays in timely administration of appropriate antibiotic therapy lead to increased patient mortality, poor clinical outcomes and use of broad-spectrum antibiotics. To overcome this delay in reporting antibiotic susceptibility results we must pursue technologies that can expedite AST results to enable appropriate and timely therapies